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Affordable Plant Tissue Culture Part 2: Orchids |
Carol M. Stiff, Kitchen Culture Kits, Inc., Lufkin, TX
www.kitchenculturekit.com
kck@turbonet.com
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Knudson C Medium with Sucrose: _____ 1 packet Knudson C medium _____ 1 ml PPM _____ 5 teaspoons sugar IF MEDIA DOES NOT INCLUDE SUCROSE Prepare media according to instructions in manual: Add ingredients to 1 quart or 1 liter of filtered or distilled water. Mix well. Adjust pH to 5.5. Measure out 3 tablespoons to a baby food jar. Add agar to each jar before processing. Sterilize media as described in manual. 1/2 MS Medium with Sucrose: _____ 1 packet MS basal medium with vitamins (no sucrose) _____ 1 ml PPM _____ 4 tablespoons sugar Prepare media according to instructions in manual: Add ingredients to 2 quarts or 2 liters of filtered or distilled water. Mix well. Adjust pH to 5.5. Measure out 3 tablespoons to a baby food jar. Add agar to each jar before processing. Sterilize media as described in manual. |
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© MMIV - Volume 1 Number 5 Whole Number 5 Tropical Visions September 2004